• Characterization of Apoptosis Using Phosphorylated Gamma-H2AX and DNA Content

• Multi-Parametric High Content Cellular Analysis of Cell Cycle Progression and Cleavage of Caspase-3 during Camptothecin-Induced Apoptosis

• Automated Laser Scanning Imaging Cytometry in Apoptotic Pathway Characterization

• Transfluor^® Assay on the iCyte^® Imaging Cytometer

• Further Transfluor^® Assay Development on the iCyte^® Imaging Cytometer

• Quantitative Cell Cycle Information Compared to Cytochalasin B Blockage in Cell Line Micronucleus Assays

• Mitochondrial-Specific Agents for Effective Targeting of Tumor Cells

• Poster: High-Content Analysis of Differential Effects of Camptothecin on Cell Lines: Apoptosis vs. Senescence

• Detection of Differential Effects of Camptothecin on the Cell Cycle

• Measurement of G2/M Index by Immunostaining of Phosphorylated Histone H3 (Ser 10)

• Combined Fluorescence and Chromatic Based Hepato-Pathological Tissue Analysis

• Tools for High-Content Automated Tissue Analysis

• High-Content Analysis of Tissue Microarrays: Extending the Genomics Revolution to Tissue Analysis

• Automated Molecular Analysis Of Chromatically And Fluorescently Stained Microarrays

• Automated Quantitative Assessment Of HER 2 Protein Expression In Tissue Microarrays By Laser Scanning Cytometry: Correlation With Visual Grading And Fish HER 2 Copy Number

• Automated Analysis of Chromatically Stained Tissue Sections by Three Laser (RBG) Scanning Cytometry

• LSC Technology Basics and Comparison with Other Cytometry Technologies

• Laser Scanning Cytometry Technology

• Applications of Laser Scanning Cytometry in Preclinical Drug Safety Studies, Drug Discovery Technology 2004

If you are interested in receiving one of CompuCyte’s presentations, please send a request to CompuCyte using the Publication Request Form.

• Utilization of Chromagenic Dyes for Quantitative Tissue Analysis:  David Krull, Research Scientist, Molecular and Ultrastructural Pathology, GlaxoSmithKline Safety Assessment, Research Triangle Park, NC, CompuCyte Workshop, CA, 2005

• Use of the iCyte^® Automated Imaging Cytometer for enhanced high-content cellular analysis approaches and screening applications: Stephan Ruetz, Lab Head, Oncology Research, Novartis, Switzerland, CompuCyte Workshop, CA, 2005

• Mitochondrial-specific agents for effective targeting of tumor cells: Shazib Pervaiz, Vice Dean (Research and Graduate Studies) and Director, Faculty of Medicine, National University of Singapore, CompuCyte Workshop, CA, 2005

• High Throughput, High Content Screening with Fixed, Archival Tissues: James F. Eliason, Chief Scientific Officer, Asterand, Detroit, MI, CompuCyte Workshop, CA, 2005

• High Content vs. High Throughput: You Can’t Have One Without the Other, Raffi Manoukian, Amgen, ISAC 2004.

• FCCP- Induced Toxicity and Oxidative Stress in Rhabdomyosarcoma Cells, Catherine Xu at al., Glaxo SmithKline, Society for Toxicology Annual Meeting 2003

• 6-Hydroxydopamine Induces Oxidative Stress and Toxicity in Human Neuroblastoma  Cells,  Catherine Xu at al., Glaxo SmithKline, Society for Toxicology Annual Meeting 2004

• Evaluation of Specific Gene Silencing Using siRNA in Single Cells by iCyte Imaging Cytometry, Stephan Ruetz, Novartis, ScreenTech 2004

• Quantum Nanoparticles and Laser Scanning Cytometry:  William Telford, Ph.D., Head, NCI ETI Branch Flow Cytometry Core Laboratory, NIH, Bethesda, MD., CBR Symposium, 2005

• Cytometry of DNA Damage:  Zbigniew Darzynkiewicz, M.D., Ph.D., Director, Brander Cancer Research Institute, New York Medical College, Hawthorne, NY.,  CBR Symposium, 2005

• Analysis of Fluorogenic Enzyme Systems on the Laser Scanning Cytometer. William Telford, NCI/NIH

• Analysis of Cytolytic T Cell Activity Using a Fluorogenic Caspase 6 Probe on the Laser Scanning Cytometer, William Telford, NCI/NIH

• Detection of HIV-1 Viral Fusion Using a Fluorogenic Beta-lactamase Reporter System on the Laser Scanning Cytometer, William Telford, NCI/NIH

• Analysis of Cell Migration through Micropore Membranes by Laser Scanning Cytometer, William Telford, NCI/NIH

• Measurement of Markers of Hepatotoxicity in Formalin-fixed Tissues Using Laser Scanning Cytometer, Dominique Brees, Pfizer, Drug Discovery Technology Congress 2004

If you are interested in receiving one of CompuCyte’s presentations, please send a request to CompuCyte using the Publication Request Form.

• Cell-Cycle Based Live-Cell Toxicological Studies

• Characterization of DNA Strand Breaks Using Phosphorylated Histone Gamma-H2AX and DNA content.

• Multi-fluorescent Application Using Hoechst 33342, Mitoshift, Yo-Pro-1 and PI to Determine Cell Viability, Function and Cytotoxicity.

• Comparison of DRAQ5 and Hoechst 33342 Fluorescent Dyes for Staining Live Cell Nuclei

• Measurement of Intracellular Signal Transduction Activity Level by Phosphorylated ERK 1/2  Immunostaining

• Immunophenotyping Protocol for iCyte^® and iCys^® Imaging Cytometers

• Using Virtual Channels to Perform Compensation and Correct Background Autofluorescence in Laser Scanning Cytometry

• Protein Expression in situ on Tissue Arrays

• Analysis of Chromatically Stained Tissue Arrays

• Cap Survey HER2 Immunohistochemistry

• Dye Selection Application Notes

• Cell Count Application Notes

If you are interested in receiving one of CompuCyte’s iGeneration Assay Protocols, please send a request to CompuCyte using the Publication Request Form.

These sample applications were originally developed for the LSC; however, they can be applied to the iCyte and iCys instruments.

• Apoptosis, annexin V

• Apoptosis, BrdUTP

• Apoptosis, general

• Bacterial Detection

• Comet Assay

• Cytoplasmic B1

• DNA Content, bladder

• DNA Content, breast

• DNA Content, paraffin embedded

• Drug toxicology

• FISH Probes

• Immunophenotyping plus DNA

• Intracellular Protein Detection

• Platelet adhesion, endothelial cells

• Tissue Mapping, dermal

• Tissue Mapping, TUNEL