CompuCyte Published Materials
Brochures
LSC Technology Brochure (PDF)
iBrowser Data Integration Software and iNovator Application Development Toolkit Brochure (PDF)
Specification Sheets
iCys Performance Specifications (PDF)
iCyte Specification Sheet (PDF)
iCys Specification Sheet (PDF)
iColor Specification Sheet (PDF)
Application Notes & Posters for iCyte and iCys Imaging Cytometers
 
 Cell-Based Applications: 
  • Characterization of Apoptosis Using Phosphorylated Gamma-H2AX and DNA Content

  • Multi-Parametric High Content Cellular Analysis of Cell Cycle Progression and Cleavage of Caspase-3 during Camptothecin-Induced Apoptosis

  • Automated Laser Scanning Imaging Cytometry in Apoptotic Pathway Characterization

  • Transfluor Assay on the iCyte Imaging Cytometer

  • Further Transfluor Assay Development on the iCyte Imaging Cytometer

  • Quantitative Cell Cycle Information Compared to Cytochalasin B Blockage in Cell Line Micronucleus Assays

  • Mitochondrial-Specific Agents for Effective Targeting of Tumor Cells

  • Poster: High-Content Analysis of Differential Effects of Camptothecin on Cell Lines: Apoptosis vs. Senescence

  • Detection of Differential Effects of Camptothecin on the Cell Cycle

  • Measurement of G2/M Index by Immunostaining of Phosphorylated Histone H3 (Ser 10)

 
Tissue-Based Applications:
  • Combined Fluorescence and Chromatic Based Hepato-Pathological Tissue Analysis

  • Tools for High-Content Automated Tissue Analysis

  • High-Content Analysis of Tissue Microarrays: Extending the Genomics Revolution to Tissue Analysis

  • Automated Molecular Analysis Of Chromatically And Fluorescently Stained Microarrays

  • Automated Quantitative Assessment Of HER 2 Protein Expression In Tissue Microarrays By Laser Scanning Cytometry: Correlation With Visual Grading And Fish HER 2 Copy Number

  • Automated Analysis of Chromatically Stained Tissue Sections by Three Laser (RBG) Scanning Cytometry

 
 
CompuCyte LSC Technology Presentations 
  • LSC Technology Basics and Comparison with Other Cytometry Technologies

  • Laser Scanning Cytometry Technology

  • Applications of Laser Scanning Cytometry in Preclinical Drug Safety Studies, Drug Discovery Technology 2004

If you are interested in receiving one of CompuCyte’s presentations, please send a request to CompuCyte using the Publication Request Form.

 
End-User Presentations

iCyte and iCys Presentations
  • Utilization of Chromagenic Dyes for Quantitative Tissue Analysis:  David Krull, Research Scientist, Molecular and Ultrastructural Pathology, GlaxoSmithKline Safety Assessment, Research Triangle Park, NC, CompuCyte Workshop, CA, 2005

  • Use of the iCyte Automated Imaging Cytometer for enhanced high-content cellular analysis approaches and screening applications: Stephan Ruetz, Lab Head, Oncology Research, Novartis, Switzerland, CompuCyte Workshop, CA, 2005

  • Mitochondrial-specific agents for effective targeting of tumor cells: Shazib Pervaiz, Vice Dean (Research and Graduate Studies) and Director, Faculty of Medicine, National University of Singapore, CompuCyte Workshop, CA, 2005

  • High Throughput, High Content Screening with Fixed, Archival Tissues: James F. Eliason, Chief Scientific Officer, Asterand, Detroit, MI, CompuCyte Workshop, CA, 2005

  • High Content vs. High Throughput: You Can’t Have One Without the Other, Raffi Manoukian, Amgen, ISAC 2004.

  • FCCP- Induced Toxicity and Oxidative Stress in Rhabdomyosarcoma Cells, Catherine Xu at al., Glaxo SmithKline, Society for Toxicology Annual Meeting 2003

  • 6-Hydroxydopamine Induces Oxidative Stress and Toxicity in Human Neuroblastoma  CellsCatherine Xu at al., Glaxo SmithKline, Society for Toxicology Annual Meeting 2004

  • Evaluation of Specific Gene Silencing Using siRNA in Single Cells by iCyte Imaging Cytometry, Stephan Ruetz, Novartis, ScreenTech 2004

 
 LSC presentations
  • Quantum Nanoparticles and Laser Scanning Cytometry:  William Telford, Ph.D., Head, NCI ETI Branch Flow Cytometry Core Laboratory, NIH, Bethesda, MD., CBR Symposium, 2005

  • Cytometry of DNA Damage:  Zbigniew Darzynkiewicz, M.D., Ph.D., Director, Brander Cancer Research Institute, New York Medical College, Hawthorne, NY.,  CBR Symposium, 2005

  • Analysis of Fluorogenic Enzyme Systems on the Laser Scanning Cytometer. William Telford, NCI/NIH

  • Analysis of Cytolytic T Cell Activity Using a Fluorogenic Caspase 6 Probe on the Laser Scanning Cytometer, William Telford, NCI/NIH

  • Detection of HIV-1 Viral Fusion Using a Fluorogenic Beta-lactamase Reporter System on the Laser Scanning Cytometer, William Telford, NCI/NIH

  • Analysis of Cell Migration through Micropore Membranes by Laser Scanning Cytometer, William Telford, NCI/NIH

  • Measurement of Markers of Hepatotoxicity in Formalin-fixed Tissues Using Laser Scanning Cytometer, Dominique Brees, Pfizer, Drug Discovery Technology Congress 2004

 

If you are interested in receiving one of CompuCyte’s presentations, please send a request to CompuCyte using the Publication Request Form.

iGeneration Assay Protocols
Step-by-step instructions for performing specific applications on iGeneration instruments

  • Cell-Cycle Based Live-Cell Toxicological Studies

  • Characterization of DNA Strand Breaks Using Phosphorylated Histone Gamma-H2AX and DNA content.

  • Multi-fluorescent Application Using Hoechst 33342, Mitoshift, Yo-Pro-1 and PI to Determine Cell Viability, Function and Cytotoxicity.

  • Comparison of DRAQ5 and Hoechst 33342 Fluorescent Dyes for Staining Live Cell Nuclei

  • Measurement of Intracellular Signal Transduction Activity Level by Phosphorylated ERK 1/2  Immunostaining

  • Immunophenotyping Protocol for iCyte and iCys Imaging Cytometers

  • Using Virtual Channels to Perform Compensation and Correct Background Autofluorescence in Laser Scanning Cytometry

  • Protein Expression in situ on Tissue Arrays

  • Analysis of Chromatically Stained Tissue Arrays

  • Cap Survey HER2 Immunohistochemistry

  • Dye Selection Application Notes

  • Cell Count Application Notes

 

If you are interested in receiving one of CompuCyte’s iGeneration Assay Protocols, please send a request to CompuCyte using the Publication Request Form.

Application Notes for the LSC

These sample applications were originally developed for the LSC; however, they can be applied to the iCyte and iCys instruments.

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