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Leipzig April 2006 Presentations

Quantitative Determination and Clinical Utility of the Analysis of Circulating Epithelial Tumor Cells in Peripheral Blood: Prof. Dr. med. Katharina Pachmann, Friedrich Schiller University Jena, Dr. med. Ulrich Pachmann, Transfusion Medical Center Bayreuth, Germany

Clinically Relevant Cellular Activation Markers:  Maurice R.G. O’Gorman, Professor Pediatrics and Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA

Effect of Selenium on Mitochondrial Genetic and Metabolic Stress in Human Osteosarcoma Cells: Joanna Szczepanowska, et al., Nenki Institute of Experimental Biology, Warsaw, Poland

From Basic Science to Clinical Diagnostics–A Field Report: Schwock J, Hedley DW, and Geddie WR, Department of Laboratory Medicine and Pathobiology, Princess Margaret Hospital, Ontario Cancer Institute, University of Toronto, Ontario, Canada

Multi-parameter Analysis of Cell Death Signaling in Cancer Cells Shazib Pervaiz, MD, PhD, National University of Singapore

Selected LSC Applications: 1Zsolt Bacso, 2Attila Megyeri, 3James F. Eliason,1University of Debrecen, Medical and Health Science Center, Department of Biophysics and Cell Biology,2University of Debrecen, Medical and Health Science Center, Department of Pharmacology and Pharmacotherapy,3Barbara Ann Karmanos Cancer Institute, Detroit, Michigan, USA

Slide Based Cytometry or How to Measure Anything in a Cell: Attila Tarnok, Pediatric Cardiology, Cardiac Center, University of Leipzig, Germany

Application Notes for iCyte and iCys Imaging Cytometers
Cell-Based Applications
Characterization of Apoptosis Using Phosphorylated Gamma-H2AX and DNA Content

Multi-Parametric High Content Cellular Analysis of Cell Cycle Progression and Cleavage of Caspase-3 during Camptothecin-Induced Apoptosis

Automated Laser Scanning Imaging Cytometry in Apoptotic Pathway Characterization

Transfluor® Assay on the iCyte™ Imaging Cytometer

Further Transfluor® Assay Development on the iCyte™ Imaging Cytometer

Quantitative Cell Cycle Information Compared to Cytochalasin B Blockage in Cell Line Micronucleus Assays

Mitochondrial-Specific Agents for Effective Targeting of Tumor Cells

Poster: High-Content Analysis of Differential Effects of Camptothecin on Cell Lines: Apoptosis vs. Senescence

Detection of Differential Effects of Camptothecin on the Cell Cycle

Measurement of G2/M Index by Immunostaining of Phosphorylated Histone H3 (Ser 10)

Tissue-Based Applications

Combined Fluorescence and Chromatic Based Hepato-Pathological Tissue Analysis

 Tools for High-Content Automated Tissue Analysis
High-Content Analysis of Tissue Microarrays: Extending the Genomics Revolution to Tissue Analysis
 Automated Molecular Analysis Of Chromatically And Fluorescently Stained Microarrays
Automated Quantitative Assessment Of Her2 Protein Expression In Tissue Microarrays By Laser Scanning Cytometry: Correlation With Visual Grading And Fish Her2 Copy Number
Automated Analysis of Chromatically Stained Tissue Sections by Three Laser (RBG) Scanning Cytometry
CompuCyte LSC Technology Presentations 

LSC Technology Basics and Comparison with Other Cytometry Technologies

Laser Scanning Cytometry Technology

Applications of Laser Scanning Cytometry in Preclinical Drug Safety Studies, Drug Discovery Technology 2004

End-User Presentations
iCyte and iCys Presentations

Utilization of Chromagenic Dyes for Quantitative Tissue Analysis:  David Krull, Research Scientist, Molecular and Ultrastructural Pathology, GlaxoSmithKline Safety Assessment, Research Triangle Park, NC, CompuCyte Workshop, CA, 2005

Use of the iCyte® Automated Imaging Cytometer for enhanced high-content cellular analysis approaches and screening applications: Stephan Ruetz, Lab Head, Oncology Research, Novartis, Switzerland, CompuCyte Workshop, CA, 2005

Mitochondrial-specific agents for effective targeting of tumor cells: Shazib Pervaiz, Vice Dean (Research and Graduate Studies) and Director, Faculty of Medicine, National University of Singapore, CompuCyte Workshop, CA, 2005

High Throughput, High Content Screening with Fixed, Archival Tissues: James F. Eliason, Chief Scientific Officer, Asterand, Detroit, MI, CompuCyte Workshop, CA, 2005

High Content vs. High Throughput: You Can’t Have One Without the Other, Raffi Manoukian, Amgen, ISAC 2004

FCCP- Induced Toxicity and Oxidative Stress in Rhabdomyosarcoma Cells, Catherine Xu at al., Glaxo SmithKline, Society for Toxicology Annual Meeting 2003

6-Hydroxydopamine Induces Oxidative Stress and Toxicity in Human Neuroblastoma  CellsCatherine Xu at al., Glaxo SmithKline, Society for Toxicology Annual Meeting 2004

Evaluation of Specific Gene Silencing Using siRNA in Single Cells by iCyte Imaging Cytometry, Stephan Ruetz, Novartis, ScreenTech 2004

LSC presentations

Quantum Nanoparticles and Laser Scanning Cytometry:  William Telford, Ph.D., Head, NCI ETI Branch Flow Cytometry Core Laboratory, NIH, Bethesda, MD. 

Cytometry of DNA Damage:  Zbigniew Darzynkiewicz, M.D., Ph.D., Director, Brander Cancer Research Institute, New York Medical College, Hawthorne, NY. 

Analysis of Fluorogenic Enzyme Systems on the Laser Scanning Cytometer, William Telford, NCI/NIH

Analysis of Cytolytic T Cell Activity Using a Fluorogenic Caspase 6 Probe on the Laser Scanning Cytometer, William Telford, NCI/NIH

Detection of HIV-1 Viral Fusion Using a Fluorogenic Beta-lactamase Reporter System on the Laser Scanning Cytometer, William Telford, NCI/NIH

Analysis of Cell Migration through Micropore Membranes by Laser Scanning Cytometer, William Telford, NCI/NIH

Measurement of Markers of Hepatotoxicity in Formalin-fixed Tissues Using Laser Scanning Cytometer, Dominique Brees, Pfizer, Drug Discovery Technology Congress 2004

iGeneration Assay Protocols

Characterization of DNA Strand Breaks Using Phosphorylated Histone Gamma-H2AX and DNA content

Multi-fluorescent Application Using Hoechst 33342, Mitoshift, Yo-Pro-1 and PI to Determine Cell Viability, Function and Cytotoxicity
Comparison of DRAQ5 and Hoechst 33342 Fluorescent Dyes for Staining Live Cell Nuclei
Measurement of Intracellular Signal Transduction Activity Level by Phosphorylated ERK 1/2  Immunostaining
Immunophenotyping Protocol for iCyte™ and iCys™ Imaging Cytometers
Using Virtual Channels to Perform Compensation and Correct Background Autofluorescence in Laser Scanning Cytometry
Protein Expression in situ on Tissue Arrays
Analysis of Chromatically Stained Tissue Arrays
Cap Survey HER2 Immunohistochemistry
Dye Selection Application Notes
Cell Count Application Notes
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