| LSC Configurations |
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| The following standard configurations are available for the LSC: |
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Description |
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250-3371-000 (110VAC)
250-3371-001 (220VAC)
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LSC® Laser Scanning Cytometer Basic System
Argon Laser, 2 PMTs, Computer with 20” Flat Screen Monitor, WinCyte ® Software
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250-2501-000 (110VAC)
250-2501-001 (220VAC)
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LSC® Laser Scanning Cytometer with 2 Lasers
Argon (488nm) and Helium-Neon (633nm) lasers, 4 PMTs, Epi-fluorescence Light, Color Camera and Monitor, Computer with 20” Flat Screen Monitor, WinCyte ® Software
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250-3151-000 (110VAC)
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LSC® with 3 Lasers
Argon (488nm), Helium-Neon (633nm) and Violet 405nm) lasers, 4 PMTs, Epi-fluorescence Light, Color Camera and Monitor, Computer with 20” Flat Screen Monitor, WinCyte ® Software
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| LSC Options and Peripherals |
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| In addition to these LSC-specific options, see Filter Sets. |
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| LSC Hardware Options |
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| LSC Computer and Software Options |
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| LSC Remote Workstation Option |
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| LSC Violet Laser Option |
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The 30mW diode laser operating at 405nm excites the most popular traditional UV dyes, like DAPI and Hoechst variants.
Detection wavelengths are in the 445-485nm range.
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Cell Cycle and CTN Examples
Hoechst 33342
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| Laser-Scan Imaging with Precision 5-Phase Autostage Option |
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| Laser-scan images are not taken with a digital camera. The high-quality images seen in these samples are generated by the scanning laser beam of the LSC. No special software or system adjustments are necessary to produce these images, as would be necessary with a digital or film camera. Instead, the pictures are generated with the same instrument settings as were used for the cytometric data acquisition. |
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| LSC Near-Infrared (IR) Option |
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| The optional near-IR optics expand the capabilities of the LSC to allow the use of fluorochromes that have emission spectra in the 750-800nM range. This section describes two protocols using the near-IR option on the LSC Laser Scanning Cytometer. |
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| Protocol 1: Adherent HeLa cells stained with violet, blue, and red laser-excitable dyes |
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| This protocol was designed to analyze stained adherent HeLa cells as follows: |
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| Events were segmented on DNA content. |
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At right, the CompuColor scan data display showing DNA (Hoechst blue), beta tubulin (FITC green), and cytokeratin (Cy5 red) staining |
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The same scan data display with CompuColor settings changed to show Pyronin Y presence in the nucleoli. |
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Protocol 2:
Adherent HeLa cells stained for RNA and DNA using Pyronin Y and the near IR emitting DRAQ-5 |
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| This protocol was designed to analyze stained adherent HeLa cells as follows: |
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Events were segmented on the virtual channel signal (ADDER) consisting
of the summation of Orange and Near IR signals |
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At right, the CompuColor scan data display showing the DNA (DRAQ 5) and the segmenting contours derived from the Adder channel. |
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The same scan field as depicted by the signals from the orange detector, indicating the Pyronin Y stained RNA. |
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CompuColor composite scan data display with laser scatter imaging. |
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