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iBrowser® Data Integration Software |
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With the iGeneration applications, various types of data files, both numerical and image, can be defined and saved. Each type of data element may have several entities, and iCyte and iCys can obtain a complete set of the data elements for all the samples in an analysis, such as wells in a microtiter plate analysis or core samples in a tissue microarray.
iBrowser® Data Integration Software provides a convenient method to access, review, and report on data.
A Run file serves as a “master index” for all data associated with a given run. iBrowser uses the Run file as the key to displaying, analyzing, and reporting on iCyte or iCys Run data.
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| Displaying Summary Data |
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| The iBrowser software displays summary data for a specific Run, including annotations for that Run, the Run name and ID, and a table of the well features for all wells or scan areas for that Run. Figure 1 shows the portion the Run file for an experiment in the starting window of the iBrowser software. iBrowser displays the experimental annotations, as well as the actual Well Feature data. |
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| Figure 1: iBrowser Summary Data |
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| Displaying Carrier Data |
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| Carrier data is data that is relevant to an experiment as a whole. iBrowser displays each available element in one of a series of tabs. Within each tab the data element is laid out to approximate the physical arrangement of the wells or scan areas for the carrier used for that Run – for example, scan images (shown in CompuColor) and histograms: |
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| Depending on the data saved during a Run, the following information can be viewed in iBrowser: |
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- Well images
- Scan field images
- Galleries of cells
- Histograms
- Scattergrams
- Two-parameter histograms
- Kolmogorov-Smirnov statistical analysis
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iBrowser provides a modified form of the Kolmogorov-Smirnov test, in which a group of control wells is defined. The test compares each of the histograms in the data set to the control value and generates a new D-value histogram.
Data generated on multiple carriers within a single run (using the iCyte robot plate handler) may be displayed one of two ways. Data may be displayed on a carrier-by-carrier basis, with the user selecting which carrier’s data to display for each data tab. Alternatively, all the wells or scan areas may be displayed together. This latter option allows comparisons between wells of different carriers.
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| Displaying Well Data |
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Any of the Well Features generated in iCys or iCyte can be graphed as well feature histograms, scattergrams or as 2-parameter histograms (expression maps).
A group of wells or a scan area may be defined so that specified statistics may be applied to data from the entire group. Groups may be substituted for individual wells in the well-data display such that graphical data of an average of the group members (as opposed to a single well) is displayed.
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| Reporting iCyte and iCys Findings |
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| iBrowser can generate reports from any tab. Additionally, using the Well Report feature, a report can be generated that contains multiple elements for a single well, such as Well Images, Galleries, and Scattergrams. For example: |
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| Reports can be printed, saved as images, or saved into PDF files. |
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