| Cell Cycle and DNA Content Applications |
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| Introduction |
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| Cell cycle analysis was one of the earliest applications developed on the LSC platform and continues to be one of the most important. As in flow cytometry, the total amount of DNA per cell can be precisely and stoichiometrically determined to obtain cell cycle distributions. In addition, one of the morphometric features obtained for segmented nuclei, the MaxPixel, is directly correlated with condensation of chromatin in nuclei and can be used to differentiate interphase cells from mitotic cells. This is accomplished with a single biomarker and a single detector, a significant benefit of LSC over flow cytometry, freeing detectors for quantifying other biomarkers. |
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| Highlights of Analysis with iCyte™, iCys™, and iColor Imaging Cytometers |
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| Automated Analysis |
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| The cell cycle may be resolved based on DNA content and the morphometric parameter MaxPixel. |
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| The cell types falling into specific regions may be viewed as elements in a gallery. The cells shown below fall into the 'mitotic' region. |
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| Using the automation enhancements and additional software capabilities of iGeneration platforms, the DNA content of various populations can be compared. |
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| Kolmogorov-Smirnov D-Value histograms highlight the drug effects. Drug-Treated Cells are shown in box |
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| As with the LSC, iGeneration systems may be used to detect and quantify aneuploid populations. For information about the detecting aneuploid populations with the LSC, see LSC Application Notes. |
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| Proliferating Cells |
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| In heterogeneous cell populations, where some cells are proliferating and others are in a G0 state, proliferation markers may be used and detected to identify the subpopulation of proliferating cells. Suitable markers detectable on the iGeneration platforms include the cyclins, histone post-translational modifications (e.g., phosphorylation of histone H3) and ki67. |
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| Benefits of Using iGeneration Technology |
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| Multiple probes can be analyzed in a single sample while obtaining widefield images, which may be correlated to the quantitative data in galleries or field images. The iGeneration platform combines the advantages of flow cytometry and image analysis in a single system. The iGeneration inverted format supports a diversity of carrier types, allowing flexibility in matching the needs of your application with the appropriate carrier type. |
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| More Information |
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| For more information, review the CompuCyte Published Materials and CompuCyte Bibliography. |
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