iGeneration Applications Overview

CompuCyte's iGeneration of instruments allows analysis of a diversity of sample types including fixed cells, live cells, tissue samples (including sections or touch preparations) and tissue arrays.

The descriptions and links below provide an overview of applications well-suited for the iGeneration systems.

 
Cellular Analysis  Applications
  • Cell Viability, Proliferation and Function

    • Cell Cycle

  • Viability and cytotoxicity  (nuclear size, cell membrane integrity, mitochondrial potential, cell proliferation)

Mitochondrial-Specific Agents for Effective Targeting of Tumor Cells

  • Apoptosis

Automated Laser Scanning Imaging Cytometer in Apoptotic Pathway Characterization

Multi-Parametric High Content Cellular Analysis of Cell Cycle Progression and Cleavage of Caspase-3 During Camptothecin-Induced Apoptose

Characterization of Apoptosis Using Phosphorylated Gamma-H2AX and DNA Content

Poster: High-Content Analysis of Differential Effects of Camptothecin on Cell Lines: Apoptosis vs. Senescence

Characterization of DNA Strand Breaks Using Phosphorylated Histone Gamma-H2AX and DNA content

  • DNA damage and repair

Characterization of Apoptosis Using Phosphorylated Gamma-H2AX and DNA Content

Quantitative Cell Cycle Information Compared to Cytochalasin B Blockage in Cell Line Micronucleus Assays

Cytometric assessment of ATM activation, histone H2AX phosphorylation and induction of apoptosis in response to DNA damage; relation to the cell cycle phase

Analysis of Cellular DNA Content by Flow and Laser Scanning Cytometry

  • Translocation assays

Transfluor® Assay on the iCyte™ Imaging Cytometer

Automated Laser Scanning Imaging Cytometry in Apoptotic Pathway Characterization

  • Reporter assays

Automated Laser Scanning Imaging Cytometry in Apoptotic Pathway Characterization

  • Live Cell Assays

Cell-Cycle Based Live-Cell Toxicological Studies

  • Cell structure

    • Cell-cell interactions

    • Lipids

    • Nucleus and Nucleoli

    • Peroxisomes

  • Immunophenotyping

Cytology and Laser Scanning Cytometry Wintrobe's Atlas of Clinical Hematology 

Koss' Diagnostic Cytology and Its Histopathologic Bases   

Cell Surface Immunophenotyping   

Tissue Immunophenotyping   

  • Cell Signaling

Measurement of Intracellular Signal Transduction Activity Level by Phosphorylated ERK 1/2  Immunostaining

  • siRNA applications

The cytotoxic ribonuclease onconase targets RNA interference (siRNA)

 
Tissue Analysis  Applications
  • Automated tissue analysis

    • IHC (including chromogenic and fluorescent detection)

  • ISH (FISH and CISH)

Quantification of Telomere Length by Laser Scanning Cytometry

 

Applied Areas of Research (reference peer-reviewed publications)

  • Biomarker discoveries

Quantitation of Caspase3 Activation as a Pharmacodynamic Endpoint in Fine Needle Aspirate (FNA) Biopsies

Validation of a Laser Scanning Cytometer for Use in Regulated Studies

Experimental Validation of Peptide Immunohistochemistry Controls

  • Stem cell research

Spatial analysis of hematopoietic stem and progenitor cells in bone marrow

  • Rare cell analysis and circulating tumor cells

Circulating epithelial tumor cells in the metastatic pathway

  • Islet viability assessment

Comprehensive analysis of human pancreatic islets using flow and laser scanning cytometry

Characterization of pancreatic ductal cells in human islet preparations

Glucose-Stimulated Increment in Oxygen Consumption Rate as a Standardized Test of Human Islet Quality

c-Jun N-terminal kinase 1 is deleterious to the function and survival of murine pancreatic islets.

Improvement of Canine Islet Yield by Donor Pancreas Infusion With a p38MAPK Inhibitor

Improvement of Human Islet Cryopreservation by a p38 MAPK Inhibitor

  • Genomic health

  • Utilization of Novel Dyes

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